Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: (A) CXCL8 and (B) IL-6 production from epithelial cells stimulated with BCG in combination with IL-17A and/or IFN-γ for 0, 24, 48 and 72 hours, MOI 1:1. The 72 hours time points are in addition represented as bar graphs for (C) CXCL8 and (D) IL-6. The results are depicted as mean ± SD of three experiments. Means were compared with one way ANOVA followed by multiple comparisons test with Tukey’s correction and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001.

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques:

Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: Primary neutrophils were stimulated with BCG for one, two and three hours in the presence of IL-17A and/or IFN-γ. (A) NETs were studied in a light microscope after 2 hours and stained with DAPI and (B) large NET structures were observed in addition to single NETs. (C) Quantification of NETs was performed with Picogreen assay on cell supernatants after 3 hours and (D) over time at 1, 2 and 3 hours. Results are depicted as mean ± SD from a total of 4 donors. Means of samples were were compared with negative control using ANOVA followed by Dunnet’s multiple comparisons test and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001. Bars represent 25 μm (A, B left panel) or 100 μm (B, right panel)

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques: Light Microscopy, Staining, Picogreen Assay, Negative Control

Journal: Journal of Traditional and Complementary Medicine

Article Title: Polysaccharides derived from alkali-extracted vinegar-baked Radix Bupleuri suppress hyperimmune T lymphocytes and ameliorate skin graft rejection

doi: 10.1016/j.jtcme.2024.11.006

Figure Lengend Snippet: The effect of KVBCP3 and KVBCP4 on immune cytokines and Treg cells of mice with skin allograft rejection. (A) The secretion of IL-2 in serum was determined by Elisa assay (n = 6). (B) The secretion of IL-4 in serum was determined by Elisa assay (n = 6). (C) Representative flow cytometry plots of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day; (D) The change of the proportions of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day (n = 3). (E) Representative flow cytometry plots of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day; (F) The change of the proportions of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day (n = 3). ∗ P < 0.05, ∗∗ P < 0.01 vs Model group, one way ANOVA.

Article Snippet: Cell stimulation and Protein Transport Inhibitor Kit (Lot: AK22752), FITC Anti-Mouse CD4 Antibody (Lot: AF21880), PE Anti-Mouse IL-17A Antibody (Lot: AF17079) were purchased from Elabscience (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry